Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PDPK1 in samples. An antibody specific for PDPK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDPK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDPK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDPK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:PDPK1 is a master kinase, which is crucial for the activation of AKT/PKB and many other AGC kinases including PKC, S6K, SGK. An important role for PDPK1 is in the signalling pathways activated by several growth factors and hormones including insulin signalling. Mice lacking PDPK1 die during early embryonic development, indicating that this enzyme is critical for transmitting the growth-promoting signals nescessary for normal mammalian development.
Mice that are deficient in PDPK1 have a 40% decrease in body mass, mild glucose intolerance, and are resitant to cancer brought about by hyperactivation of the PI3K pathway (PTEN +/-).The structure of PDPK1 can be divided into two domains; the kinase or catalytic domain and the PH domain.
