Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PDGF-AA in samples. An antibody specific for PDGF-AA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDGF-AA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDGF-AA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDGF-AA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The receptor for PDGF, PDGFR is classified as a receptor tyrosine kinase (RTK), a type of cell surface receptor. Two types of PDGFRs have been identified: alpha type and beta type PDGFRs. The alpha type binds to PDGF-AA, PDGF-BB and PDGF-AB while the beta type PDGFR binds with high affinity to PDGF-BB and PDGF-AB. PDGF binds to PDGFRs ligand binding pocket located within the second and third immunoglobulin domains. Upon activation by PDGF, these receptors dimerise, and are "switched on" by auto-phosphorylation of several sites on their cytosolic domains, which serve to mediate binding of cofactors and subsequently activate signal transduction, for example, through the PI3K pathway. The role of PI3K has been investigated by several laboratories. Accumulating data suggests that while this molecule is generally part of growth signaling complex, it plays a more profound role in controlling cell migration.
