Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PDGF in samples. An antibody specific for PDGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDGF is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Platelet-derived growth factor (PDGF) is one of the numerous growth factors, or proteins that regulate cell growth and division. In particular, it plays a significant role in blood vessel formation (angiogenesis), the growth of blood vessels from already-existing blood vessel tissue. Uncontrolled angiogenesis is a characteristic of cancer. In chemical terms, platelet-derived growth factor is a dimeric glycoprotein composed of two A (-AA) or two B (-BB) chains or a combination of the two (-AB).
PDGF is a potent mitogen for cells of mesenchymal origin, including fibroblasts, smooth muscle cells and glial cells. In both mouse and human, the PDGF signalling network consists of four ligands, PDGFA-D, and two receptors, PDGFRalpha and PDGFRbeta. All PDGFs function as secreted, disulphide-linked homodimers, but only PDGFA and B can form functional heterodimers.
Though PDGF is synthesized,[3] stored (in the alpha granules of platelets), and released by platelets upon activation, it is also produced by a plethora of cells including smooth muscle cells, activated macrophages, and endothelial cells
