Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PDCD5 in samples. An antibody specific for PDCD5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDCD5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDCD5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDCD5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:PDCD5 encodes a protein expressed in tumor cells during apoptosis independent of the apoptosis-inducing stimuli. Prior to apoptosis induction, this gene product is distributed in both the nucleus and cytoplasm. Once apoptosis is induced, the level of this protein increases and by relocation from the cytoplasm, it accumulates in the nucleus. Although its exact function is not defined, this protein is thought to play an early and universal role in apoptosis.This TFAR19 gene encodes a protein of 125 amino acids, with an ATG initiation codon at nucleotides 25-27. It contains a cAMP- and cGMP-dependent protein kinase phosphorylation site, multiple protein kinase C phosphorylation sites, and a casein kinase II phosphorylation site. It shares significant homology to the corresponding proteins of species ranging from yeast to mice.
