Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PCSK6 in samples. An antibody specific for PCSK6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCSK6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCSK6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCSK6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Proprotein convertase subtilisin/kexin type 6 is an enzyme that belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. This encoded protein is a calcium-dependent serine endoprotease that can cleave precursor protein at their paired basic amino acid processing sites. Each PACE4 isoform exhibits a unique restricted distribution. PACE4A-I is expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, but at comparatively higher levels in the liver. PACE4A-II is at least expressed in placenta. PACE4B was only found in the embryonic kidney cell line from which it was isolated. PACE4C and PACE4D are expressed in placenta.
