Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PALM in samples. An antibody specific for PALM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPALM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PALM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PALM bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Paralemmin is a member of the paralemmin protein family. PALM has a highly conserved coiled-coil N terminus that displays a periodicity of acidic, basic, hydrophobic, and glutamine residues, as well as 2 potential leucine zippers.
PALM has a central motif similar to a sequence found in lipid-anchored SNAREs that is involved in endoplasmic reticulum-Golgi transport. The C terminus of PALM contains a cluster of basic residues, which likely contribute to membrane association, putative palmitoylated cysteines, and a prenylation consensus motif. PALM also has phosphorylation motifs for several serine/threonine kinases. The 5-prime untranslated regions of the chicken, mouse, and human transcripts are GC rich.
