Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate PABPC1 in samples. An antibody specific for PABPC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPABPC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PABPC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PABPC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The poly(A)-binding protein (PAB or PABP), which is found complexed to the 3' poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation. In humans, the PABPs comprise a small nuclear isoform and a conserved gene family that displays at least 3 functional proteins: PABP1 (PABPC1), inducible PABP (iPABP, or PABPC4; MIM 603407), and PABP3 (PABPC3; MIM 604680). In addition, there are at least 4 pseudogenes, PABPCP1 to PABPCP4.[supplied by OMIM]
