Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate MCAM in samples. An antibody specific for MCAM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMCAM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCAM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCAM bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:CD146 (cluster of differentiation 146) is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. In humans, the CD146 protein is encoded by the MCAM gene.
Its function is still poorly understood, but evidence points to it being part of the endothelial junction associated with the actin cytoskeleton. A member of the Immunoglobulin superfamily, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. It is expressed on chicken embryonic spleen and thymus, activated human T cells, endothelial progenitors such as angioblasts and mesenchymal stem cells, and strongly expressed on blood vessel endothelium and smooth muscle.
