Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate MAZ in samples. An antibody specific for MAZ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAZ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAZ is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAZ bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The MYC gene product functions as a DNA-specific transcription factor. A related polypeptide called MAX , for MYC-associated factor X, dimerizes with MYC to optimize DNA binding. A paucity of negative regulatory elements have been reported for the MYC gene which regulate transcription from 2 initiation sites specified by the P1 and P2 promoters. ME1a1, a 16-bp nuclear nuclear factor binding site residing between MYC P1 and P2 transcription initiation sites, is required for P2 activity. MAZ, Its mRNA was present in all tissues tested (except kidney) as a doublet of approximately 2.5 to 2.7 kb, along with differentially expressed minor species. The authors speculated that MAZ may encode a transcription factor with dual roles in transcription initiation and termination.
