Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate MAVS in samples. An antibody specific for MAVS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAVS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAVS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAVS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:These interactions initiate signaling pathways that differ in their initial steps but converge in the activation of the protein kinases IKKA (CHUK) and IKKB (IKBKB), which activate NFKB , or TBK1 and IKKE (IKBKE), which activate IRF3. Activated IRF3 and NFKB induce transcription of IFNB (IFNB1). For the TLR3 pathway, the intermediary molecule before the pathways converge is the cytoplasmic protein TRIF (TICAM1). For RIGI, the intermediary protein is mitochondria-bound IPS1 (Sen and Sarkar, 2005).Present in T-cells, monocytes, epithelial cells and hepatocytes (at protein level). Ubiquitously expressed, with highest levels in heart, skeletal muscle, liver, placenta and peripheral blood leukocytes.
