Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate MATK in samples. An antibody specific for MATK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMATK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MATK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MATK bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:MATKis an enzyme has amino acid sequence similarity to Csk tyrosine kinase and has the structural features of the CSK subfamily: SRC homology SH2 and SH3 domains, a catalytic domain, a unique N terminus, lack of myristylation signals, lack of a negative regulatory phosphorylation site, and lack of an autophosphorylation site.This protein is thought to play a significant role in the signal transduction of hematopoietic cells. It is able to phosphorylate and inactivate Src family kinases, and may play an inhibitory role in the control of T-cell proliferation. This protein might be involved in signaling in some cases of breast cancer. Three alternatively spliced transcript variants that encode different isoforms have been described for this gene.
