• Product NameOsteopontin Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Purificationprotein G purifed
• ApplicationsWB IHC
• Species ReactivityHu
• Specificityspecific for human Osteopontin denatured and native forms
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant Human Osteopontin
• Target NameOsteopontin
• Alternative Names    Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, SPP-1, Urinary stone protein, Uropontin, BNSP, OPN, PSEC0156
• Accession No. Swiss-Prot#: P10451
• UniprotP10451
• Gene ID6696;
• Calculated MW 34.5kd
• Concentration 1.0mg/mL
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  Immunohistochemistry: 1:20 - 1:200

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity

[1] Kiefer M.C., Bauer D.M., Barr P.J. The cDNA and derived amino acid sequence for human osteopontin. Nucleic Acids Res. 17:3306-3306(1989) [2] Young M.F., Kerr J.M., Termine J.D., Wewer U.M., Wang M.G., McBride O.W., Fisher L.W. cDNA cloning, mRNA dist

• All Lanes Mouse anti human OPN monoclonal antibody at at 2 ug/ml Lane 1 mouse kidney tissue lysate Secondary Goat polyclonal to Mouse IgG at 1/5000 dilution Predicted band size : 34.5 kDa Observed band size: 35kDa Additional bands at : 50 kDa. We are unsure as to the identity of this extra band.
• Immunohistochemical analysis of paraffin-embedded human colon cancer using #42036 at dilution of 1:200.
• Immunohistochemical analysis of paraffin-embedded human Kidney tissue using #42036 at dilution of 1:200.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting