Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate MAST3 in samples. An antibody specific for MAST3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAST3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAST3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAST3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:By sequencing clones obtained from a size-fractionated brain cDNA library, Nagase et al. (1998) cloned MAST3, which they designated KIAA0561. The transcript contains repetitive elements in its 3-prime UTR, and the deduced 1,308-amino acid protein shares significant similarity with mouse Mast205 (MAST2) and human MAST4. RT-PCR detected variable MAST3 expression in all tissues examined, with highest levels in kidney, brain, and small intestine.Valiente et al. (2005) showed that the C-terminal tail of human PTEN bound to the PDZ domains of rat Magi2, Magi3, and Dlg (DLG1), mouse Sast (MAST1) and Mast205, and human MAST3. Interaction of PTEN with Magi2 increased PTEN protein stability, and interaction of PTEN with the MAST kinases facilitated phosphorylation of PTEN by these kinases.
