Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate MASP2 in samples. An antibody specific for MASP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMASP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MASP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MASP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:MASP2 is a protein involved in the complement system. Mannose-binding lectin (MBL) is a serum component which participates in innate immunity by activating complement via a novel pathway. Human MBL forms complexes with two types of serine proteases termed MASP (MBL-associated serine protease). These two proteases, MASP1 and MASP2, are structurally similar to one another as well as to C1r and C1s. Together, MASP, C1r and C1s constitute a novel serine protease family. It is likely that human MASP1 is able to activate C3, while human MASP2 cleaves C4, although further functional studies are required to confirm this. Based on the analysis of MASP cDNA of vertebrates and ascidians, the MASP/C1r/C1s family can be classified into two groups.
