• Product NameCardiac Troponin I Monoclonal Antibody
• Host SpeciesMouse
• ClonalityMonoclonal
• Purificationprotein G purifed
• ApplicationsWB
• Species ReactivityHu
• SpecificityReacts equally with free cardiac troponin I and cardiac troponin I forming complexes with other troponin components. Not affected by heparin, phosphorylation and oxidation. No cross-reactivity with skeletal muscle troponin I.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant Human Cardiac Troponin I protein
• Target NameCardiac Troponin I
• Alternative Names    CTnI
• Accession No. Swiss-Prot#: P19429
• UniprotP19429
• Gene ID7137;
• Calculated MW 23kd
• Concentration 1.0mg/mL
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  

Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC). Each subunit is responsible for part of troponin complex function. TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles. Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium.

[1] Apple FS.Tissue specificity of cardiac troponin I,cardiac troponin T and creatine Kinase-MB[J].Clin Chim Acta,1999,284:151-159 [2] Stein PD, Janjua M, Matta F, Pathak PK, Jaweesh F, Alrifai A, Chughtai HL. Prognosis based on creatine kinase isoenzyme

• All lanes : Mouse monoclonal to Human Cardiac Troponin I at 1ug/ml Line 1:rat heart muscle lysate Line2:mouse heart muscle lysate Line3:CTnI transfected SF9 cell lysate Predicted band size : 23kd Observed band size : 30kd

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting