Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate MAPK13 in samples. An antibody specific for MAPK13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAPK13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPK13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPK13 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Mitogen Activated Protein Kinase 13 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development.
This kinase is closely related to p38 MAP kinase, both of which can be activated by proinflammatory cytokines and cellular stress. MAP kinase kinases 3, and 6 can phosphorylate and activate this kinase. Transcription factor ATF2, and microtubule dynamics regulator stathmin have been shown to be the substrates of this kinase.Expressed in testes, pancreas, small intestine, lung and kidney. Abundant in macrophages, also present in neutrophils, CD4+ T-cells, and endothelial cells.
