Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate MAP1LC3B2 in samples. An antibody specific for MAP1LC3B2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAP1LC3B2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAP1LC3B2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAP1LC3B2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:MAP1LC3b2 Belongs to the MAP1 LC3 family.MAP1LC3b is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.The deduced 125-amino acid protein shares 94% identity with rat Map1lc3. Northern blot analysis detected transcripts of 2.5 and 1.0 kb abundantly expressed in heart, brain, skeletal muscle, and testis, with weaker expression in all other tissues examined. MAP1LC3B was posttranslationally modified. Unlike MAP1LC3A and MAP1LC3C, however, MAP1LC3B did not undergo C-terminal cleavage and did not require the conserved gly120 for posttranslational modification.
