Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate MAO in samples. An antibody specific for MAO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAO is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAO bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:This gene encodes monoamine oxidase A, an enzyme that degrades amine neurotransmitters, such as dopamine, norepinephrine, and serotonin. The protein localizes to the mitochondrial outer membrane. The gene is adjacent to a related gene on the opposite strand of chromosome X. Mutation in this gene results in monoamine oxidase deficiency, or Brunner syndrome.
