Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate FCN1 in samples. An antibody specific for FCN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFCN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FCN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FCN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. The collagen-like and the fibrinogen-like domains are also found separately in other proteins such as complement protein C1q, C-type lectins known as collectins, and tenascins. However, all these proteins recognize different targets, and are functionally distinct.Ficolin 1 encoded by FCN1 is predominantly expressed in the peripheral blood leukocytes, and has been postulated to function as a plasma protein with elastin-binding activity.
