Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate MAEL in samples. An antibody specific for MAEL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAEL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAEL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAEL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:By excision of a P element insertion in the 5-prime UTR of the Drosophila maelstrom gene, Findley et al. (2003) isolated mael(M391), a null allele of Drosophila maelstrom. They used database analysis to identify the human MAEL homolog. Confocal microscopy localized Drosophila maelstrom primarily to nuage, highly abundant particles within germline cells, and also to the nucleus and cytoplasm of germline cells. Nuclear shuttling assays showed that Drosophila maelstrom is transported between the cytoplasm and nucleus.By phenotypic characterization of Drosophila maelstrom mutants, which displayed axial patterning defects and other polarity defects, Findley et al. (2006) defined maelstrom as a spindle-class gene that affects Vasa modification.
