Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate MACC1 in samples. An antibody specific for MACC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMACC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MACC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MACC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:MACC1 is a key regulator of the hepatocyte growth factor (HGF)-HGF receptor (HGFR, or MET) pathway, which is involved in cellular growth, epithelial-mesenchymal transition, angiogenesis, cell motility, invasiveness, and metastasis. Expression of MACC1 in colon cancer specimens is an independent prognostic indicator for metastasis formation and metastasis-free survival.The deduced 852-amino acid has an SH3 domain, a proline-rich motif for binding SH3 domain-containing proteins, additional binding motifs for SH2 and SH3 domain-containing proteins, EPS15 homology domains, clathrin and retinoblastoma protein (RB1) interaction domains, and putative tyrosine phosphorylation sites. It shares about 44% identity with SH3BP4, and putative orthologs were identified in chicken, mouse, rat, dog, and chimp.
