Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate M6PR in samples. An antibody specific for M6PR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyM6PR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for M6PR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of M6PR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:M6PR is a receptor for mannose-6-phosphate groups on lysosomal enzymes. The receptor forms a homodimer or homotetramer for intracellular targeting of lysosomal enzymes and export of newly synthesized lysosomal enzymes into the cell secretions. The receptor is an integral membrane protein which localizes to the trans-Golgi reticulum, endosomes, and the plasma membrane. Two distinct mannose 6-phosphate receptors have been identified: one is cation-independent and has an apparent molecular weight of 215,000. The second, the cation-dependent mannose 6-phosphate receptor, or small mannose 6-phosphate receptor, has an apparent molecular weight of only 46,000 and requires divalent cations for high-affinity binding with its ligand.
