Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate LY75 in samples. An antibody specific for LY75 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLY75 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LY75 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LY75 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The LY75 protein has 77% overall amino acid identity with mouse Dec205. LY75 is a member of the MMR family of multidomain molecules. It contains a signal peptide, a cysteine-rich domain, a fibronectin type II domain, 10 carbohydrate recognition-like domains, a transmembrane domain, and a cytoplasmic tail. Northern blot analysis of hematopoietic cell lines revealed the presence of a predominant 7.8-kb and a secondary 9.5-kb transcript in myeloid, B-cell, and Hodgkin cell lines but not in the Jurkat T-cell line. In peripheral blood, immature DC contained barely detectable transcripts, which increased 50-fold upon differentiation and activation.LY75 acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment.
