Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate LPL in samples. An antibody specific for LPL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLPL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LPL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism
