Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate LMAN2 in samples. An antibody specific for LMAN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLMAN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LMAN2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LMAN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:By searching an EST database for sequences similar to dog Vip36, Neve et al. (2003) identified LMAN2, which they called VIP36. The deduced 356-amino acid protein contains an N-terminal signal sequence, followed by a lectin-type carbohydrate recognition domain and a C-terminal transmembrane domain. A KRFY endoplasmic reticulum trafficking motif is located at the C terminus. Northern blot analysis detected variable expression of 1.2- and 1.4-kb VIP36 transcripts in all tissues examined. Highest expression was in kidney, placenta, and liver.
Nufer et al. (2003) determined that the LMAN2 gene contains 8 exons and spans 14.9 kb.By genomic sequence analysis, Nufer et al. (2003) mapped the LMAN2 gene to chromosome 5q35.5.
