Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate LIPH in samples. An antibody specific for LIPH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLIPH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LIPH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LIPH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Lipase member H is a membrane-bound member of the mammalian triglyceride lipase family. It catalyzes the production of 2-acyl lysophosphatidic acid (LPA), which is a lipid mediator with diverse biological properties that include platelet aggregation, smooth muscle contraction, and stimulation of cell proliferation and motility.Present in intestine (at protein level). Expressed in colon, prostate, kidney, pancreas, ovary, testis, intestine, lung and pancreas. Expressed at lower level in brain, spleen and heart. In hair, it is prominently expressed in hair follicles, including the stem cell-rich bulge region.Defects in LIPH are the cause of alopecia universalis congenita Mari type (AUCM). AUCM is a recessive autosomal form of alopecia that is common in the Mari population, a large aboriginal Finno-Ugric population in the Volga-Ural region of Russia.
