Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate LIPE in samples. An antibody specific for LIPE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLIPE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LIPE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LIPE bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Gastric lipase is an acidic lipase secreted by the gastric chief cells in the fundic mucosa in the stomach. It has a pH optimum of 3-6. Gastric lipase, together with lingual lipase, comprise the two acidic lipases. These lipases, unlike alkaline lipases (such as pancreatic lipase), do not require bile acid or colipase for optimal enzymatic activity. Acidic lipases make up 30% of lipid hydrolysis occurring during digestion in the human adult, with gastric lipase contributing the most of the two acidic lipases. In neonates, acidic lipases are much more important, providing up to 50% of total lipolytic activity.Gastric lipase is a polypeptide of 371 residues in length. The structure of gastric lipase was determined using X-ray diffraction with a resolution of 3.00 ?, and is composed of 41% helices and 14% beta sheets.
