Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate LGI3 in samples. An antibody specific for LGI3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLGI3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LGI3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LGI3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The deduced 549-amino acid protein contains a N-terminal signal peptide, a putative transmembrane region, and 1 partial and 4 complete leucine-rich repeats (LRRs). The LRRs are flanked on both sides by cysteine-rich regions, and the N-terminal cysteine-rich region contains a motif conserved in LGIs. LGI3 shares significant similarity with LGI1, LGI2, and LGI4. Semiquantitative PCR detected LGI3 expression in all tissues examined, with highest levels in brain and lung.
By in situ hybridization of adult mouse brain, Senechal et al. (2005) showed that Lgi3 was expressed at low levels in most brain regions with high levels in the facial nerve nucleus. Lgi3 was secreted in transfected 293T cells.
