Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate LGALS4 in samples. An antibody specific for LGALS4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLGALS4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LGALS4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LGALS4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Galectin-4 belongs to a subfamily of galectins composed of two carbohydrate recognition domains within the same peptide chain. The two domains have all the conserved galectin signature amino acids, but their overall sequences are only approximately 40% identical. Both domains bind lactose with a similar affinity as other galectins, but their respective preferences for other disaccharides, and larger saccharides, are distinctly different. Thus galectin-4 has a property of a natural cross-linker, but in a modified sense since each domain prefers a different subset of ligands. Similarly to other galectins, galectin-4 is synthesized as a cytosolic protein, but can be externalized. During development and in adult normal tissues, galectin-4 is expressed only in the alimentary tract, from the tongue to the large intestine.
