Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate LDLRAP1 in samples. An antibody specific for LDLRAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLDLRAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LDLRAP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LDLRAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:LDLRAP1 is a protein encoded by this gene is a cytosolic protein which contains a phosphotyrosine binding (PTD) domain. The PTD domain has been found to interact with the cytoplasmic tail of the LDL receptor. Mutations in this gene lead to LDL receptor malfunction and cause the disorder autosomal recessive hypercholesterolaemia.Expressed at high levels in the kidney, liver, and placenta, with lower levels detectable in brain, heart, muscle, colon, spleen, intestine, lung, and leukocytes.Adapter protein (clathrin-associated sorting protein (CLASP)) required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). May be required for LDL binding and internalization but not for receptor clustering in coated pits.
