Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate LCP1 in samples. An antibody specific for LCP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLCP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LCP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LCP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.).
L-plastin has been found in many types of malignant human cells of non-hemopoietic origin suggesting that its expression is induced accompanying tumorigenesis in solid tissues.
