Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate LAMC2 in samples. An antibody specific for LAMC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLAMC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAMC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAMC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:LAMC2 is the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly thought to be a truncated version of beta chain (B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5, which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized.
