Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate LAMB2 in samples. An antibody specific for LAMB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLAMB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAMB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAMB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Laminin subunit beta-2 is the beta chain isoform laminin, beta 2. The beta 2 chain contains the 7 structural domains typical of beta chains of laminin, including the short alpha region. However, unlike beta 1 chain, beta 2 has a more restricted tissue distribution. It is enriched in the basement membrane of muscles at the neuromuscular junctions, kidney glomerulus and vascular smooth muscle. Transgenic mice in which the beta 2 chain gene was inactivated by homologous recombination, showed defects in the maturation of neuromuscular junctions and impairment of glomerular filtration. Alternative splicing involving a non consensus 5' splice site (gc) in the 5' UTR of this gene has been reported. It was suggested that inefficient splicing of this first intron, which does not change the protein sequence, results in a greater abundance of the un.
