Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate LALBA in samples. An antibody specific for LALBA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLALBA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LALBA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LALBA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Alpha-lactalbumin is an important whey protein in cow's milk (~1 g/l), and is also present in the milk of many other mammalian species. In primates, alpha-lactalbumin expression is upregulated in response to the hormone prolactin and increases the production of lactose. Alpha-lactalbumin forms the regulatory subunit of the lactose synthase (LS) heterodimer and beta-1,4-galactosyltransferase (beta4Gal-T1) forms the catalytic component. Together, these proteins enable LS to produce lactose by transferring galactose moieties to glucose. As a monomer, alpha-lactalbumin strongly binds calcium and zinc ions and may possess bactericidal or antitumor activity. A folding variant of alpha-lactalbumin, called HAMLET, likely induces apoptosis in tumor and immature cells.
