Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate KTN1 in samples. An antibody specific for KTN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKTN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KTN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KTN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Kinectin is a protein encoded by the KTN1 gene.Various cellular organelles and vesicles are transported along the microtubules in the cytoplasm. Likewise, membrane recycling of the endoplasmic reticulum (ER), Golgi assembly at the microtubule organizing center, and alignment of lysosomes along microtubules are all related processes.
The transport of organelles requires a special class of microtubule-associated proteins (MAPs). One of these is the molecular motor kinesin, an ATPase that moves vesicles unidirectionally toward the plus end of the microtubule. Another such MAP is kinectin, a large integral ER membrane protein. Antibodies directed against kinectin have been shown to inhibit its binding to kinesin.
