Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate KRT17 in samples. An antibody specific for KRT17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKRT17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT17 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT17 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Keratin 17 is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. Mutations in the gene encoding this protein lead to Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex.The functional KRT17 gene differs from the pseudogenes by the extent of methylation of certain DNA sequences in the 5-prime upstream region. The KRT17 gene encodes a polypeptide of 432 amino acids with a calculated molecular weight of 48,000. Using S1-nuclease protection assays and RNAs from several cell lines, Troyanovsky et al. identified a single transcriptional start point 26 nucleotides downstream from a TATA box element. Synthesis of cytokeratin-17 seems to be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial 'stem cell.'
