Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate KLK13 in samples. An antibody specific for KLK13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLK13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLK13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLK13 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Kallikrein-13 is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Expression of this gene is regulated by steroid hormones and may be useful as a marker for breast cancer. An additional transcript variant has been identified, but its full length sequence has not been determined.
in a 300-kb region on chromosome 19q13.3-q13.4, where other kallikrein genes are localized. KLK13 exists in 2 main forms: a long form that encodes a deduced 277-amino acid protein with a predicted molecular mass of 30.6 kD, and a short form, referred to as the short KLKL4 variant, that uses an upstream alternative splice donor site located in exon 3 that generates a truncated protein. The KLK13 protein shares 51% amino acid identity with KLK11 and KLK6.
