Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate KLK12 in samples. An antibody specific for KLK12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLK12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLK12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLK12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Kallikrein-12 is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in three transcript variants encoding different isoforms.In the second mRNA form, the last exon is split into 2 separate exons with an additional intervening intron, producing a deduced 254-amino acid protein with a predicted molecular mass of 27.1 kD. In the third mRNA form, the fourth exon is missing, producing a deduced 111-amino acid protein with a molecular mass of 12 kD. RT-PCR demonstrated that KLK12 is expressed primarily in salivary gland, stomach, uterus, trachea, prostate, thymus, lung, colon, brain, breast, and thyroid gland and at lower levels in testis, pancreas, small intestine, and spinal cord.
