Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate KLK11 in samples. An antibody specific for KLK11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLK11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLK11 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLK11 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Kallikrein 11 is a member of the human kallikrein gene family. KLK11 is a secreted serine protease, highly expressed in hormonally regulated tissues, including the prostate and the ovary. KLK11 has two alternative splicing isoforms, known as the brain type and prostate type. Many members of the human kallikrein gene family are differentially expressed in cancer and a few have potential as diagnostic/prognostic markers. KLK11 may represent a diagnostic and prognostic biomarker for ovarian cancer. KLK11 expression in prostate, testis, trachea, pancreas, salivary gland, thyroid, parathyroid, lung, stomach, and adult and fetal skin. Weaker expression was detected in heart, mammary gland, uterus, colon, and small intestine, with little to no expression in other tissues examined.
