Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate KLK10 in samples. An antibody specific for KLK10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLK10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLK10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLK10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:KLK10 is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its encoded protein is secreted and may play a role in suppression of tumorigenesis in breast and prostate cancers. Alternate splicing of this gene results in multiple transcript variants encoding the same protein.
The deduced 276-amino acid protein contains a putative signal peptide, followed by a short activating peptide and the protease domain, which includes the catalytic triad of his86, asp137, and ser229. KLK10 also contains 12 conserved cysteines in its protease domain that are expected to form 6 disulfide bonds, as in other serine proteases. RT-PCR detected variable levels of KLK10 expression in nearly all of the 35 adult and fetal tissues examined.
