Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate KLF5 in samples. An antibody specific for KLF5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLF5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLF5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLF5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Krueppel-like factor 5 is a member of the Kruppel-like factor subfamily of zinc finger proteins. Since the protein localizes to the nucleus and binds the epidermal growth factor response element, it is thought to be a transcription factor.
Klf5, also known as (IKLF) or (Bteb2) has been assigned purely transcriptional activation activity (Fig. 1A) but, similar to Klf4, binds p300 which acetylates the first zinc finger conferring a trans-activating function. Importantly for Klf4 & Klf5, the amino acids that are predicted by the Klevit model to interact with DNA are identical (Fig. 1B and Fig. 2) and the two compete for the same CACCC element found in a wide variety of promoters.
