Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate KLF2 in samples. An antibody specific for KLF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLF2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Mature single-positive (CD4+ or CD8+) T lymphocytes enter a 'resting' state in which they are proliferatively quiescent and relatively resistant to apoptosis. Anderson et al. (1995) identified and characterized the gene for a novel zinc finger transcription factor that they symbolized LKLF for 'lung Kruppel-like factor.'
Using 5-prime and 3-prime RACE on a melted GC-rich DNA fragment similar to mouse Lklf, Kozyrev et al. (1999) isolated a cDNA encoding KLF2, which they termed LKLF. Sequence analysis predicted that the 355-amino acid LKLF protein, which is 87% identical to the mouse protein, has N-terminal proline-rich repeats, a putative activation domain, a potential nuclear localization signal, and a C-terminal zinc finger domain.
