Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate KLF15 in samples. An antibody specific for KLF15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLF15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLF15 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLF15 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:KLF15 protein is 84% identical to the rat Klf15 protein, contains 3 zinc finger motifs at its C terminus, N-terminal serine-rich stretches, and a central proline-rich segment. EMSA analysis confirmed that KLF15 and MAZ interact with the GA element of the CLCNKA promoter and showed that KLF15 binds with higher affinity and is a functional competitor of MAZ. Northern blot analysis revealed highest expression of a 2.5-kb KLF15 transcript in liver, followed by heart, skeletal muscle, and kidney. No expression was found in bone marrow or lymphoid tissues. KLF15 was also expressed in cardiac and skeletal muscle interstitial cells and in kidney inner medulla, glomeruli, and cortical interstitium. Immunofluorescence microscopy, however, demonstrated no colocalization of KLF15 with CLCNKA.
