Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate KIT in samples. An antibody specific for KIT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKIT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KIT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KIT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Stem cell factor Receptor(SCFR;CD117), also called KIT or C-kit receptor, is a cytokine receptor expressed on the surface of hematopoietic stem cells as well as other cell types. This receptor binds to stem cell factor (a substance that causes certain types of cells to grow). Altered forms of this receptor may be associated with some types of cancer.C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in its encoded gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.
