Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate KIFC2 in samples. An antibody specific for KIFC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKIFC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KIFC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KIFC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:KIFC2, a novel kinesin superfamily member from mouse brain. KIFC2 encodes a 792 amino acid protein, which contains the conserved motor domain at the C-terminal end of the protein and is most similar to members of the KAR3 family involved in cell division. Expression analysis localized KIFC2 mRNA to nonproliferative neuronal cells in the central nervous system, and immunolocalization studies demonstrated that KIFC2 is present in axons and dendrites of neurons in the central and peripheral nervous systems. KIFC2 localizes with some, but not all, axonally transported organelles. Finally, ligation of mouse peripheral nerves showed that KIFC2 accumulates at the proximal and distal sides of an axonal ligature. KIFC2 may play a role in retrograde axonal transport.
