Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate KIF18A in samples. An antibody specific for KIF18A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKIF18A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KIF18A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KIF18A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:KIF18A contains a motor domain and conserved ATP- and microtubule-binding sites, and the central region is predicted to have an alpha-helical coiled-coil, followed by a C-terminal cargo-binding domain. Nuclear localization signals, protein-protein interaction motifs such as SH3, WW, AP-1, and FHA domains, and potential sites for phosphorylation and sumoylation were also identified. KIF18A shares 84% and 81% homology with the mouse and rat homologs, respectively. Indirect immunofluorescence microscopy localized endogenous KIF18A to the nucleus, cytoplasm, and plasma membrane ruffles in preosteogenic marrow stromal cells. Punctate cytoplasmic staining of KIF18A was associated with microtubules and partial colocalization with actin.
