Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate KHSRP in samples. An antibody specific for KHSRP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKHSRP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KHSRP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KHSRP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The KHSRP gene encodes a multifunctional RNA-binding protein implicated in a variety of cellular processes, including transcription, alternative pre-mRNA splicing, and mRNA localization. The pre-mRNA of the protooncogene SRC contains an 18-nucleotide exon, N1, that is spliced into the mRNA in neuronal cells but is excluded in nonneuronal cells. N1 exon inclusion in neurons is under the positive control of an intronic regulatory sequence called the downstream control sequence (DCS).The predicted 711-amino acid protein contains a proline/glycine-rich N terminus and a C-terminal domain that is rich in proline, glycine, alanine, and glutamine. The central region contains 4 tandemly repeated KH domains, leading the authors to rename the protein 'KH-type splicing regulatory protein' (KSRP).
