Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate KERA in samples. An antibody specific for KERA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKERA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KERA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KERA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Keratocan is a protein encoded by the KERA gene.Keratan sulfate proteoglycans (KSPGs) are members of the small leucine-rich proteoglycan (SLRP) family. KSPGs, particularly keratocan, lumican, and mimecan , are important to the transparency of the cornea.
Defects in KERA are the cause of the autosomal recessive cornea plana 2 (CNA2). In CNA2, the forward convex curvature is flattened, leading to a decrease in refraction, reduced visual activity, extreme hyperopia (usually plus 10 d or more), hazy corneal limbus, opacities in the corneal parenchyma, and marked arcus senilis (often detected at an early age). CNA2 is a rare disorder with a worldwide distribution, but a high prevalence in the Finnish population.
