Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate KDM5C in samples. An antibody specific for KDM5C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKDM5C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KDM5C is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KDM5C bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:KDM5C is a member of the SMCY homolog family and encodes a protein with one ARID domain, one JmjC domain, one JmjN domain and two PHD-type zinc fingers. The DNA-binding motifs suggest this protein is involved in the regulation of transcription and chromatin remodeling. Alternative splicing generates 2 distinct transcripts, either containing or missing 9 nucleotides, which in turn predicts 2 XE169 protein isoforms composed of 1,557 and 1,560 amino acids, respectively. XE169 is expressed in multiple human tissues, and homologous sequences exist on the human Y chromosome and in the genomes of 5 other eutherian mammals examined. RT-PCR analysis of somatic cell hybrids containing either an active or an inactive human X chromosome on a rodent background demonstrated that XE169 escapes X inactivation.
