Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate KDM4A in samples. An antibody specific for KDM4A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKDM4A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KDM4A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KDM4A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:JMJD2A encodes a deduced 1,064-amino acid protein. Like JMJD2B and JMJD2C, JMJD2A contains a JmjN domain, a JmjC domain, a JD2H domain, 2 TUDOR domains, and a bipartite nuclear localization signal that overlaps the C-terminal part of the second TUDOR domain. JmjN is a small domain found in the Jumonji family of transcription factors. JmjC is a putative enzymatic domain. JD2H is a region containing 2 cys-his clusters with weak similarity to a PHD domain.This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor.
